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| Registros recuperados : 19 | |
| 4. |  | KITZBERGER, C. S. G.; SCHOLZ, M. B. S.; SILVA, J. B. G. D.; PEREIRA, L. F. P.; BENASSI, M. T. Green coffee beans composition, roasted coffee and beverages characteristics: diversity between arabica cultivars. In: INTERNATIONAL CONFERENCE ON COFFEE SCIENCE, 24., 2012, Costa Rica. Programme & abstracts. [S.l.]: Association for Science and Information on Coffee, 2012. p. 107| Biblioteca(s): Embrapa Café. |
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| 7. | | SCHOLZ, M. B. dos S.; KITZBERGER, C. S. G.; PEREIRA, L. F. P.; DAVRIEUX, F.; POT, D.; CHARMETANT, P.; LEROY, T. Application of near infrared spectroscopy for green coffee biochemical phenotyping. Journal of Near Infrared Spectroscopy, v. 22, n. 6, p. 411-421, 2014.| Biblioteca(s): Embrapa Café. |
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| 9. | | PAGIATTO, N. F.; IVAMOTO, S. T.; SERA, T.; KITZBERGER, C. S. G.; SCHOLZ, M. B. S.; CHARMETANT, P.; LEROY, T.; DOMINGUES, D.; PEREIRA, L. F. P. Evaluation of kahweol and cafestol levels in Ethiopian Coffea arabica L. accessions grown in Brazil. In: INTERNATIONAL CONFERENCE ON COFFEE SCIENCE, 24., 2012, Costa Rica. Programme & abstracts. [S.l.]: Association for Science and Information on Coffee, 2012. p. 217| Biblioteca(s): Embrapa Café. |
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| 10. | | GARRETT, R.; SCHMIDT, E. M.; PEREIRA, L. F. P.; KITZBERGER, C. S. G.; SCHOLZ, M. B. S.; EBERLIN, M. N.; REZENDE, C. M. Discrimination of arabica coffee cultivars by electrospray ionization fourier transform ion cyclotron resonance mass spectrometry and chemometrics. LWT - Food Science and Technology, v. 50, n. 2, p. 496-502, 2013.| Biblioteca(s): Embrapa Café. |
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| 11. | | KITZBERGER, C. S. G.; SCHOLZ, M. B. dos S.; PEREIRA, L. F. P.; VIEIRA, L. G. E.; SERA, T.; SILVA, J. B. G. D.; BENASSI, M. de T. Diterpenes in green and roasted coffee of Coffea arabica cultivars growing in the same edapho-climatic conditions. Journal of Food Composition and Analysis, v. 30, n. 1, P. 52-57, 2013.| Biblioteca(s): Embrapa Café. |
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| 12. | | IVAMOTO-SUZUKI, S. T.; CELEDÓN, J. M.; YUEN, M. M. S.; KITZBERGER, C. S. G.; DOMINGUES, D. S.; BOHLMANN, J.; PEREIRA, L. F. P. Functional characterization of ent-copalyl diphosphate synthase and kaurene synthase genes from coffea arábica L. Journal of Agricultural and Food Chemistry, v. 71, n. 42, p. 15863-15873, 2023.| Biblioteca(s): Embrapa Café. |
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| 13. | | SCHOLZ, M. B. dos S.; KITZBERGER, C. S. G.; PAGIATTO, N. F.; PEREIRA, L. F. P.; DAVRIEUX, F.; POT, D.; CHARMETANT, P.; LEROY, T. Chemical composition in wild ethiopian Arabica coffee accessions. Euphytica, v. 209, n. 2 p. 429-438, 2016.| Biblioteca(s): Embrapa Café. |
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| 14. | | IVAMOTO, S. T.; KITZBERGER, C. S. G.; SCHOLZ, M. B. S.; ANDRADE, D. S.; ALVES, L. C.; DOMINGUES, D. S.; VIEIRA, L. G. E.; PEREIRA, L. F. P. In silico analysis of cytochrome p450 genes involved in the metabolism of diterpenes in Coffea. In: SIMPÓSIO BRASILEIRO DE GENÉTICA MOLECULAR DE PLANTAS, 3., 2011, Ilhéus. Resumos...| Biblioteca(s): Embrapa Café. |
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| 15. | | SCHOLZ, M. B. S.; PAGIATTO, N. F.; KITZBERGER, C. S. G.; PEREIRA, L. F. P.; DAVRIEUX, F.; CHARMETANTE, P.; LEROYE, T. Validation of near-infrared spectroscopy for the quantification of cafestol and kahweol in green coffee. Food Research International, v. 61, p. 176-182, 2014.| Biblioteca(s): Embrapa Café. |
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| 16. | | IVAMOTO, S. T.; CALEDÓN, J. M.; KITZBERGER, C. S. G.; SCHOLZ, M. B. S.; BOHLMANN, J.; DOMINGUES, D. S.; PEREIRA, L. F. P. Transcriptome analysis and characterization of genes involved in diterpene biosynthesis in coffea arabica. In: CONGRESSO BRASILEIRO DE MELHORAMENTO DE PLANTAS, 9., 2017, Foz de Iguaçu. Melhoramento de plantas: projetando o futuro. Maringá, PR: SBMP, 2017. p. 105| Biblioteca(s): Embrapa Café. |
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| 17. | | IVAMOTO, S. T.; SAKURAY, L. M.; FERREIRA, L. P.; KITZBERGER, C. S. G.; SCHOLZ, MARIA B. S.; POT, D.; LEROY, T.; VIEIRA, L. G. E.; DOMINGUES, D. S.; PEREIRA, L. F. P. Diterpenes biochemical profile and transcriptional analysis of cytochrome P450s genes in leaves, roots, flowers, and during Coffea arabica L. fruit development. Plant Physiology and Biochemistry, v. 111, p. 340-347, Dec. 2016.| Biblioteca(s): Embrapa Café. |
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| 18. | | IVOMOTO, S. T.; SAKURAY, L. M.; FERREIRA, L. P.; KITZBERGER, C. S. G.; SCHOLZ, M. B. S.; POT, D.; LEROY, T.; VIEIRA, L. G. E.; DOMINGUES, D. S.; PEREIRA, L. F. P. Diterpenes biochemical profile and transcriptional analysis of cytochrome P450s genes in leaves, roots, flowers, and during Coffea arabica L. fruit development. Plant Physiology and Biochemistry, v. 111, p. 340-347, February, 2017.| Biblioteca(s): Embrapa Café. |
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| 19. | | SANT'ANA, G. C.; PEREIRA, L. F. P.; POT, D.; IVAMOTO, S. T.; DOMINGUES, D. S.; FERREIRA, R. V.; PAGIATTO, N. F.; SILVA, B. S. R. da; NOGUEIRA, L. M.; KITZBERGER, C. S. G.; SCHOLZ, M. B. S.; OLIVEIRA, F. F. de; SERA, G. H.; PADILHA, L.; LABOUISSE, J-P.; GUYOT, R.; CHARMETANT, P.; LEROY, T. Genome-wide association study reveals candidate genes influencing lipids and diterpenes contents in Coffea arabica L . Scientific Reports, v. 8, n. 465, p. 1-12, 2018| Biblioteca(s): Embrapa Café. |
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| Registros recuperados : 19 | |
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 | Acesso ao texto completo restrito à biblioteca da Embrapa Café. Para informações adicionais entre em contato com biblioteca@embrapa.br. |
Registro Completo
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Biblioteca(s): |
Embrapa Café. |
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Data corrente: |
06/12/2023 |
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Data da última atualização: |
06/12/2023 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 1 |
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Autoria: |
IVAMOTO-SUZUKI, S. T.; CELEDÓN, J. M.; YUEN, M. M. S.; KITZBERGER, C. S. G.; DOMINGUES, D. S.; BOHLMANN, J.; PEREIRA, L. F. P. |
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Afiliação: |
SUZANA TIEMI IVAMOTO-SUZUKI, UNIVERSIDADE ESTADUAL DE LONDRINA; JOSÉ MIGUEL CELEDÓN, UNIVERSITY OF BRITISH COLUMBIA; MACAIRE M. S. YUEN, UNIVERSITY OF BRITISH COLUMBIA; CÍNTIA SORANE GOOD KITZBERGER, INSTITUTO DE DESENVOLVIMENTO RURAL DO PARANÁ; DOUGLAS SILVA DOMINGUES, ESCOLA SUPERIOR DE AGRICULTURA LUIZ DE QUEIROZ; JÖRG BOHLMANN, UNIVERSITY OF BRITISH COLUMBIA; LUIZ FILIPE PROTASIO PEREIRA, CNPCa. |
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Título: |
Functional characterization of ent-copalyl diphosphate synthase and kaurene synthase genes from coffea arábica L. |
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Ano de publicação: |
2023 |
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Fonte/Imprenta: |
Journal of Agricultural and Food Chemistry, v. 71, n. 42, p. 15863-15873, 2023. |
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Idioma: |
Inglês |
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Conteúdo: |
The biochemical profile of coffee beans translates directly into quality traits, nutraceutical and health promoting properties of the coffee beverage. Ent-kaurene is the ubiquitous precursor for gibberellin biosynthesis in plants, but it also serves as an intermediate in specialized (i.e., secondary) diterpenoid metabolism that leads to a diversity of more than 1,000 different metabolites. Nutraceutical effects on human health attributed to diterpenes include antioxidant, anticarcinogenic, and anti-inflammatory properties. Cafestol (CAF) and kahweol (KAH) are two diterpenes found exclusively in the Coffea genus. Our objective was to identify and functionally characterize genes involved in the central step of ent-kaurene production. We identified 17 putative terpene synthase genes in the transcriptome of Coffea arabica. Two ent-copalyl diphosphate synthase (CaCPS) and three kaurene synthase (CaKS) were selected and manually annotated. Transcript expression profiles of CaCPS1 and CaKS3 best matched the CAF and KAH metabolite profiles in different tissues. CaCPS1 and CaKS3 proteins were heterologously expressed and functionally characterized. CaCPS1 catalyzes the cyclization of geranylgeranyl diphosphate (GGPP) to ent-copalyl diphosphate (ent-CPP), which is converted to ent-kaurene by CaKS3. Knowledge about the central steps of diterpene formation in coffee provides a foundation for future characterization of the subsequent enzymes involved in CAF and KAH biosynthesis. |
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Thesaurus NAL: |
Bioinformatics; Biosynthesis; Caffeine; Coffee beans; Enzymes; Gene expression. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 02304naa a2200265 a 4500
001 2159212
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008 2023 bl uuuu u00u1 u #d
100 1 $aIVAMOTO-SUZUKI, S. T.
245 $aFunctional characterization of ent-copalyl diphosphate synthase and kaurene synthase genes from coffea arábica L.$h[electronic resource]
260 $c2023
520 $aThe biochemical profile of coffee beans translates directly into quality traits, nutraceutical and health promoting properties of the coffee beverage. Ent-kaurene is the ubiquitous precursor for gibberellin biosynthesis in plants, but it also serves as an intermediate in specialized (i.e., secondary) diterpenoid metabolism that leads to a diversity of more than 1,000 different metabolites. Nutraceutical effects on human health attributed to diterpenes include antioxidant, anticarcinogenic, and anti-inflammatory properties. Cafestol (CAF) and kahweol (KAH) are two diterpenes found exclusively in the Coffea genus. Our objective was to identify and functionally characterize genes involved in the central step of ent-kaurene production. We identified 17 putative terpene synthase genes in the transcriptome of Coffea arabica. Two ent-copalyl diphosphate synthase (CaCPS) and three kaurene synthase (CaKS) were selected and manually annotated. Transcript expression profiles of CaCPS1 and CaKS3 best matched the CAF and KAH metabolite profiles in different tissues. CaCPS1 and CaKS3 proteins were heterologously expressed and functionally characterized. CaCPS1 catalyzes the cyclization of geranylgeranyl diphosphate (GGPP) to ent-copalyl diphosphate (ent-CPP), which is converted to ent-kaurene by CaKS3. Knowledge about the central steps of diterpene formation in coffee provides a foundation for future characterization of the subsequent enzymes involved in CAF and KAH biosynthesis.
650 $aBioinformatics
650 $aBiosynthesis
650 $aCaffeine
650 $aCoffee beans
650 $aEnzymes
650 $aGene expression
700 1 $aCELEDÓN, J. M.
700 1 $aYUEN, M. M. S.
700 1 $aKITZBERGER, C. S. G.
700 1 $aDOMINGUES, D. S.
700 1 $aBOHLMANN, J.
700 1 $aPEREIRA, L. F. P.
773 $tJournal of Agricultural and Food Chemistry$gv. 71, n. 42, p. 15863-15873, 2023.
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